Microbiology Laboratory

Lab manager

Gordana Beg Paklar

assistant lab worker

Gordana Beg Paklar

About the lab

The microbiology laboratory research activities are focused on fundamental research in the field of microbial ecology of marine microorganisms. Research is also being carried out on allohtonous microorganisms that come from different sources into the marine environment and which may potentially be hazardous to human health.

The field of ecology of marine microorganisms includes research of microbial community structure (composition, abundances, biomass, production and activity), and the role of microbial food web in matter and energy flow through the pelagic ecosystem. As a part of the allochthonous microorganisms research scientific studies have been carried out regarding the presence and survival of fecal contamination indicators in seawater and their concentration in shellfish.
In the Laboratory of microbiology the research has been carried out in the field of microbial ecology of marine microorganisms as well as studies of allohtonous microorganisms that come from different sources into the marine environment.

The main focus of microbial ecology research includes:

  • characterization of microbial community structure by molecular methods and through monitoring of the abundance, biomass and activity of all components of the microbial microbial food web (viruses, autotrophic,heterotrophic and photoheterotrophic prokaryotes- bacteria and cyanobacteria, as well as autotrophic and heterotrophic eukaryotes-pikoeukariotes and nanoflagellates)
  • defining the trophic significance of a microbial community in the matter and energy flow in different marine ecosystems
  • identification of microbial response to different environmental conditions and / or changes in ecological conditions on the spatial and temporal scale

The study of microbial communities, their composition (abundance, biomass and activities), as well as the relationships within members of microbial community is inevitable for the understanding of several important processes:

  • Biological productivity of marine ecosystems and carbon flow between trophic levels, which is of paramount importance for the maintenance of fish stocks;
  • The fate of biogenic carbon and its sequestration in the marine environment that depends crucially on the size structure of microbial communities and the dominant type of nutrition networks,
  • Recognition of microbial response to different environmental conditions as an important descriptor of natural and anthropogenic changes in the marine environment.

As a part of the allochthonous microorganisms research, scientific research has been carried out regarding the presence and survival of fecal contamination in seawater and their concentration in shellfish. The results of these studies are essential for assessing the quality of water and marine organisms and potential risks to human health. Also, the results of these studies have direct application in assessing the impact of submarine runoff on the environment, and in better planning of the bivalve breeding technology.
The laboratory is currently carrying out research on new functional bacterial groups, diversity of bacterial communities using molecular methods as well as on the response of the microbial food web to global warming.

Staff

M
Mladen
Laboratorij za mikrobiologiju
S
Stefanija
Laboratorij za mikrobiologiju
D
Danijela
Laboratorij za mikrobiologiju
SJ
Slaven
Laboratorij za mikrobiologiju

Publications

Publikacije
Peharda M Schöne B R Black B A Corr ge T
Advances of sclerochronology research in the last decade
Published
2021
DOI
ISBN
10.1016/j.palaeo.2021.110371
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Publikacije
FERRERI R GENOVESE S BARRA M BIAGIOTTI I BOURDEIX J De FELICE A GAŠPAREVIĆ D HATTAB T IGLESIAS M JURETIĆ T LEONORI I MALAVOLTI S RAYKOV V SARAUX C TIČINA V VENTERO A BASILONE G
Variability in si e at maturity of the European anchovy Engraulis encrasicolus in the Mediterranean Sea
Published
2021
DOI
ISBN
10.12681/mms.25995
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Publikacije
BARRA M BONANNO A HATTAB T SARAUX C IGLESIAS5 M LEONORI I TIČINA V BASILONE G DE FELICE A FERRERI R MACHIAS A VENTERO A COSTANTINI I JURETIĆ T PYROUNAKI M M BOURDEIX J GAŠPAREVIĆ D KAPELONIS Z CANDUCI G GIANNOULAKI M
Effects of sampling intensity and biomass levels on the precision of acoustic surveys in the Mediterranean Sea
Published
2021
DOI
ISBN
10.12681/mms.26100
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Publikacije
BONANNO A BARRA M DE FELICE A GIANNOULAKI M IGLESIAS M LEONORI I VENTERO A ARONICA S BIAGIOTTI I TIČINA V CANDUCI G GENOVESE S
Acoustic correction factor estimate for compensating vertical diel migration of small pelagics
Published
2021
DOI
ISBN
10.12681/mms.25120
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Publikacije
BUDIŠA A PALIAGA P JURETIĆ T LUČIĆ D SUPIĆ N PASARIĆ Z DJAKOVAC T MLADINIĆ M DADIĆ V TIČINA V
Distribution diet and relationships of the invasive ctenophore Mnemiopsis leidyi with anchovies and ooplankton in the northeastern Adriatic Sea
Published
2021
DOI
ISBN
10.12681/mms.23305
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Publikacije
DE FELICE A IGLESIAS M SARAUX C BONANNO A TIČINA V LEONORI I VENTERO A HATTAB T BARRA M GAŠPAREVIĆ D BIAGIOTTI I BOURDEIX J GENOVESE S JURETIĆ T ARONICA S MALAVOLTI S
Environmental drivers influencing the abundance of round sardinella Sardinella aurita and European sprat Sprattus sprattus in different areas of the Mediterranean Sea
Published
2021
DOI
ISBN
10.12681/mms.25933
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Publikacije
HATTAB T GUCU A VENTERO A De FELICE A MACHIAS A SARAUX C GAŠPAREVIĆ D BASILONE G COSTANTINI I LEONORI I BOURDEIX J IGLESIAS M BARRA M GIANNOULAKI M FERRERI R El AYOUBI S GAŠPAREVIĆ D MALAVOLTI S GENOVESE S SOMARAKIS S JURETIĆ T TIČINA V CERTAIN G
Temperature strongly correlates with regional patterns of body si e variation in Mediterranean small pelagic fish species
Published
2021
DOI
ISBN
10.12681/mms.26525
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Publikacije
Angelini S Armelloni EN Costantini I De Felice A Isajlovic I Leonori I Manfredi C Masnadi F Scarcella G Ticina V Santojanni A
Understanding the Dynamics of Ancillary Pelagic Species in the Adriatic Sea
Published
2021
DOI
ISBN
10.3389/fmars.2021.728948
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Publikacije
Zorica B E geta Balić D Vidjak O Vuletin V Šestanovic M Isajlović I Čikeš Keč V Vrgoč N Harrod C
Diet Composition and Isotopic Analysis of Nine Important Fisheries Resources in the Eastern Adriatic Sea Mediterranean
Published
2021
DOI
ISBN
10.3389/fmars.2021.609432
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Publikacije
Uvanović H Schöne B R Markulin K Janeković I Peharda M
Venerid bivalve Venus verrucosa as a high resolution archive of seawater temperature in the Mediterranean Sea
Published
2021
DOI
ISBN
10.1016/j.palaeo.2020.110057
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Projects

Measurements

Atmosferska mjerenja
Obavljaju se mjerenja temperature zraka, vjetra, tlaka zraka, vlažnosti i drugih meteoroloških parametara na obalnim postajama
Mjerenja površinskih struja
Obavljaju se mjerenja temperature zraka, vjetra, tlaka zraka, vlažnosti i drugih meteoroloških parametara na obalnim postajama
Oceanografska mjerenja
Obavljaju se mjerenja temperature zraka, vjetra, tlaka zraka, vlažnosti i drugih meteoroloških parametara na obalnim postajama
Virtualni labaratorij
Obavljaju se mjerenja temperature zraka, vjetra, tlaka zraka, vlažnosti i drugih meteoroloških parametara na obalnim postajama

Gear

Water Purification System Milli-Q
Microcentrifuge Eppendorf 5417C
Epifluorescent microscopes
Flow Cytometer
Liquid Scintillation Analyzer TriCarb 2910TR
Water bath
Incubators
Autoclave
Incubator-shaker
Filtration apparatus
Ultrasonic probe
A precise scale
Diffusion chamber
Homogenizer
pH meter
Freezer (up to -80°C)

Computer Programs

Cage Number (HR)

Program služi za izračun broja (kružnih) kaveza potrebnih za uzgoj zadane godišnje količine proizvodnje ribe. Pored veličine kaveza, zadaje se duljina uzgojnog ciklusa (do dvije ili tri godine), način korištenja kaveza.

SettlingVel (HR)

Program za izračunavanje brzine taloženja čestica. Pored sfernog oblika čestice može se birati još devet geometrijskih tijela koja su unutar odgovarajućeg Reynoldsovog broja. Dimenzije čestica mogu se generirati slučajnim brojevima iz zadanih razdioba (Uniform, Normal, Log-Normal, Exponential, Weibull, Empirical). Generirani podaci mogu se prikazati grafički i spremiti kao text file.

VriIzmKav (HR)

Program služi za izračun broja (kružnih) kaveza potrebnih za uzgoj zadane godišnje količine proizvodnje ribe. Pored veličine kaveza, zadaje se duljina uzgojnog ciklusa (do dvije ili tri godine), način korištenja kaveza.